FIG. 1.

Interaction of Gem and 14-3-3. (A) The positions of serines subjected to mutagenesis in Gem are shown relative to the ras core, 14-3-3 binding site, and calmodulin binding site. (B) Cos-7 cells were transfected with wild-type or mutant Gem. Cell lysates were immunoprecipitated with anti-Gem monoclonal antibody and, subsequently, coprecipitated 14-3-3 was detected by Western blot analysis using anti-14-3-3β polyclonal antibody. Total Gem expression in the cell lysates was determined by Western blotting with anti-Gem polyclonal antibody.