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Binding of 14-3-3 to Gem protects it from degradation. Cos-7 cells transfected with wild-type Gem or the 14-3-3-nonbinding mutant, GemS23A, were treated with cycloheximide (10 μg/ml) for 0 to 6 h. Gem protein levels were determined by Western blotting using anti-Gem polyclonal antibody.
