TABLE 1.
Inhibitors used to screen for protein kinase-dependent pS-289 phosphorylation
| Inhibitor | Concn (μM) | Targeta |
|---|---|---|
| LY294002 | 25 | PI3K |
| Go6976 | 0.02, 1, 10 | PKCα, PKCβ1 |
| Go6850 | 10, 20 | PKC including ζ |
| H9 dihydrochloride | 1, 10 | PKA |
| H9 dihydrochloride | 200 | PKC |
| KT5720 | 0.3, 1, 10 | PKA |
| KT5823 | 1, 2 | PKG |
| Y27632 | 10 | Rok |
| PD98059 | 50 | Mek |
| Apigenin | 20, 80 | CK2 |
| KN93 | 1, 10, 30 | CAM kinase II |
| W7 | 1, 10, 30 | CaMK, MLCK |
a
Abbreviations: PI3K, PI 3-kinase; CAM, calmodulin-dependent; CaMK, calmodulin-dependent kinase; MLCK, myosin light-chain kinase.