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. 2012 Sep 10;7(9):e44552. doi: 10.1371/journal.pone.0044552

Figure 6. IL-17 neutralization reduces serum CTx level in Ovx mice and decreases RANKL expression in femur trabecular bone.

Figure 6

(A) After treatment animals were sacrificed and serum samples were collected from all the groups. C-terminal telopeptides of type I collagen (CTx) level was measured in all the groups by ELISA. ELISA was conducted in six replicates in each group. (B) mRNA level of OPG in the trabecular region of femur bone was studied in all the groups (method was explained in the material and method section). (C) mRNA level of RANKL in the trabecular region of femur bone was studied in all the groups. RNA from four femur bones was pooled for the study of OPG and RANKL in each group. Data represent three independent experiments and expressed as mean ± SEM with 95% confidence interval. Statistical analysis was performed by ANOVA method followed by the Newman–Keuls test of significance using Prism version 3.0 software.