Figure 9. NIL-17 mAb treatment to Ovx mice promotes mineral nodule formation and inhibits osteoclastogenesis in bone marrow microenvironment.

(A) Representative images show mineralization nodules formed in the BMCs harvested from the long bones of all groups of mice. (B) Quantitative measurement of mineralization nodules in all the groups were performed by using alizarin red staining. (C) Ex-vivo osteoclastogenesis was performed in BMCs of all the groups. After five days total RNA was isolated and real-time PCR was performed to assess the expression of TRAP gene. Data represent three independent experiments and expressed as mean ± SEM with 95% confidence interval. Statistical analysis was performed by ANOVA method followed by the Newman–Keuls test of significance using Prism version 3.0 software.