Figure 6. Parametric estimation of the stem cell's mechanical properties.

Displacements in the XY plane (A) and Z direction (B) were predicted computationally, using values varied parametrically (by orders of magnitude) about a known measured value for osteoblasts (900 Pa). By comparing experimentally measured displacements with displacements predicted for model cells with estimated moduli, a best estimate for the elastic and shear moduli of the model stem cell line could be made. The vertical axes are depicted in a logarithmic scale. C. Through linear interpolation, parametric estimates were made for elastic and shear modulus of the model stem cell, for flow generating a target shear of 1 dyn/cm² and for three seeding conditions (different densities). Error bars show standard errors (n = 3).