Table 1. Binding of Fab m43 to recombinant gp140s from primary isolates.
| Isolate | Clade | EC50, nM |
| Fab m43 | ||
| UG037.8 | A | 500 ± 291.5 |
| tethered 89.6 | B | 3 ± 0.8 |
| R2 | B | 3.5 ± 0.7 |
| MW965.26 | C | 12.3 ± 8.3 |
| GXC-44 | C | 1.5 ± 0.2 |
| CRII92UG024.2 | D | 5.4 ± 0.2 |
| GXE-14 | E | 1.9 ± 0.2 |
| 93BR019.10 | FB | 35.9 ± 14.3 |
| 92UG975.10 | G | 1.7 ± 0.4 |
Recombinant gp140s from primary isolates were directly coated on Maxisorp plates. Bound Fab m43 was detected by using HRP conjugates to anti-human IgG, F(ab′)2 as secondary antibody and ABTS as substrate. EC50 is the concentration at which antibody has half-maximum binding.