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. 2012 Sep 10;7(9):e44108. doi: 10.1371/journal.pone.0044108

Figure 5. Methylation status of the HRE site determines the sensitivity of MUC17 to HIF1α-induced transactivation.

Figure 5

(A) Demethylation of CpG sites in the MUC17 promoter in BxPC3 and PANC1 cells after 1 µM 5-azadC treatment. MUC17-negative/low cell lines were treated with or without 5-azadC for 7 days. The methylation status of the MUC17 promoter harboring HRE was examined by MSP. The PCR products labeled M (methylated) were amplified by methylation-specific primers, and those labeled U (unmethylated) were amplified by primers specific for unmethylated DNA. (B) Restoration of MUC17 expression in pancreatic cancer cell lines by 5-azadC treatment. Cells were treated with or without 5-azadC for 7 days. During the last 24 h, each group was cultured under normoxic (N) or hypoxic (H) conditions. MUC17 expression was examined by Western blotting.