FIG. 2.
Pre-steady-state kinetics of nucleotide incorporation by Polι. (A) The DNA substrate (template A) used in this study. (B) Preincubated Polι (130 nM) and DNA (300 nM) were rapidly mixed with 50 μM dTTP for various time intervals by using a rapid chemical quench flow instrument. The amount of product formed was graphed as a function of time, and the solid line represents the best fit of the data to the burst equation with an amplitude equal to 100 ± 7 nM, a burst rate constant equal to 0.63 ± 0.07 s−1, and a linear rate equal to 2.4 ± 0.6 nM/s.