TABLE 2.
Nucleotide incorporation by Polι on template T
| Nucleotide · template | kpol (s−1)a | KD (μM)a | kpol/KD (μM−1 s−1) | kpol/kpolb | KD/KDc | Fidelityd |
|---|---|---|---|---|---|---|
| dGTP · T | 0.054 ± 0.004 | 5.7 ± 0.7 | 9.5 × 10−3 | 0.54 | 0.12 | 0.07 |
| dATP · T | 0.029 ± 0.005 | 46 ± 15 | 6.3 × 10−4 | |||
| dTTP · T | 0.032 ± 0.002 | 61 ± 5 | 5.2 × 10−4 | 0.91 | 1.3 | 1.2 |
| dCTP · T | NDe | ND |
a
The kpol and KD values represent the averages and standard errors from three independent determinations.
b
Calculated by kpol for dATP·T divided by kpol for dNTP·T.
c
Calculated by KD for dNTP·T divided by KD for dATP·T.
d
Calculated by kpol/KD for dATP·T divided by kpol/KD for dNTP·T.
e
ND, no detectable incorporation observed.