Figure 1. Oncostatin M (OSM) as one of the highly expressed genes in mϕ from human chronic wounds.

Wound site macrophage (wound mϕ) were isolated from human subjects with chronic wounds. Matching blood monocyte derived macrophages (MDM) were obtained as described in methods from same subjects. A, Expression levels of OSM gene using GeneChip™ analysis. GeneChip™ expression values were normalized using global scaling approach. *, p<0.05 (n=3) compared to matched MDM. B, OSM expression data from GeneChip™ analysis was independently verified using ELISA. Wound site mϕ and matching MDM were cultured for 24h. OSM released by the cells in media was measured by ELISA. p<0.05 (n=3) matched MDM. C–E. OSM, PGE₂ and its metabolite 13,14-dihydro-15-keto PGE₂ (PGE-M) are abundant in fluid from human chronic wounds. Fluid was obtained from chronic wounds of human subjects. The levels of C, OSM, D, PGE₂ and E, PGE-M were determined using ELISA from chronic human wound fluid and matching plasma samples from the patients. The levels were normalized to the total albumin level in the fluid/plasma.* p<0.01 (n=15) compared to plasma. F, wound-site mϕ and matching MDMs were cultured for 24h. PGE₂ released by the cells was measured in culture media using ELISA. p<0.05 (n=3) matched MDM.