Figure 3.

Western blot analysis of HepG2 cells after combined treatment with sorafenib and VK2. (A) Sorafenib and VK2 synergistically inhibited the phosphorylation of ERK, and subsequently led to Mcl-1 downregulation and caspase-3 cleavage. p-ERK: phosphorylated ERK. c-caspase-3: cleaved caspase-3. (B) The levels of p-ERK and ERK were quantitated by densitometry, and the ratios of these two proteins are displayed. Values represent the mean±SD (n = 3). *p<0.05 vs. control or single-agent treatment. (C) Changes in p21 expression and the levels of cyclin D1 binding to p21. Representative results from three independent experiments with similar results are shown.