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Effect of PDK1 and PKCα knockdown on PKCη upregulation. (A) and (B) MCF-7 cells were transfected with the indicated siRNAs and then treated with or without 1μM PDBu, 100 nM TPA or 10 μM ILV for 16 h. Western blot analysis was performed with indicated antibodies. Actin was used as a loading control. Results are representative of 3 independent experiments.
