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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Insect Biochem Mol Biol. 2012 Jul 7;42(10):751–757. doi: 10.1016/j.ibmb.2012.07.002

Figure 1. IPPI assay outline.

Figure 1

Isomerization of IPP to DMAPP is catalyzed by IPPI. During the reaction an electrophilic attack by a proton from water occurs on the IPP double bond to yield the carbocation intermediate, afterwards the C-2 pro-R hydrogen of the intermediate is stereospecifically eliminated. DMAPP is converted to isoprene by acidic hydrolysis and dehydration using phosphoric acid. Volatile isoprene is adsorbed using a SPME fiber and detected by gas chromatography.