Constitutive interleukin protein expression plotted as a ratio to the internal control protein (IC) in the cerebral cortex and white matter of adult sheep.
Panel A shows: Representative Western immunoblots of IL-1β protein expression are shown for each study group. IL-1β protein expression in the cerebral cortex of pregnant adult sheep at 87–90 (n=6; open bar) and 135–137 (closed bar, n=6) days of gestation and non-pregnant adult sheep (gray bar, n=3) and in white matter of adult sheep at 87–90 (open bar, n=7) and 135–137 (closed bar, n=4) days of gestation; [ANOVA, main effect for group in cerebral cortex, F (2,12) =9.08, P<0.01 and in white matter [F (1,9) = 5.5, P<0.05 for white matter]. Values are m±SD, *P<0.05 versus pregnant ewe at 87–90 of gestation.
Panel B shows: Representative Western immunoblots of IL-6 protein expression are shown for each study group. IL-6 protein expression in the cerebral cortex of pregnant adult sheep at 87–90 (n=6) and 135–137 (n=6) days of gestation and non-pregnant adult sheep (n=3) and in white matter of pregnant adult sheep at 87–90 (n=7) and 135–137 (n=4) of days gestation; [ANOVA, main effect for group, F= (2,12) 21.8, P<0.001 in cerebral cortex and F(1,9) = 0.30, P=0.6 in white matter]. Symbol and statistical legends as for A.