Fig. 7.

WhiB4 regulates survival of Mtb inside macrophages. A. rIFNγ and LPS-activated Raw264.7 cells were infected with Mtb strains at a moi of 10 and growth was monitored over time by cfu. B and C. To investigate the role of O₂ tension, phorbol 12-myristate 13-acetate (PMA)-stimulated THP-1 human monocytic cell lines were maintained at 20% O₂ (B) and 5% O₂ (C) and infected by various Mtb strains at a moi of 10. For each strain, the cfu at each time point are expressed relative to the cfu at time 0. In each case, data shown are the average of three experiments performed in triplicate; the error bars indicate standard deviation in each group.