Figure 2. US-induced phosphorylation of Src at tyrosine 416.
Serum-deprived primary human chondrocytes were treated with US for three minutes, then total cell lysates were collected 5, 15, 30, and 60 minutes after US treatment. Total cell lysate for control was collected from chondrocytes that did not receive US treatment. Phosphorylation of Src at tyrosine 416 and total Src were demonstrated by Western blotting (A). Western blotting data from four separate experiments were quantified using Image J and averages are shown as the ratio of phosphorylated to total Src (B).