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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Ultrasound Med Biol. 2012 Aug 21;38(10):1734–1743. doi: 10.1016/j.ultrasmedbio.2012.06.002

Figure 4. US-induced phosphorylation of CrkII at tyrosine 221.

Figure 4

Serum-deprived primary human chondrocytes were treated with US for three minutes, then total cell lysates were collected 5, 15, 30 and 60 minutes after US treatment. Total cell lysate for control was collected from chondrocytes that did not receive US treatment. Phosphorylation of CrkII at tyrosine 221, total CrkII and β-actin loading control were demonstrated by Western blotting (A). Western blotting data from three separate experiments were quantified using Image J and averages are shown as the ratio of phosphorylated to total CrkII (B). # p < 0.05 vs. control.