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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Microbiol. 2012 Jul 25;85(6):1179–1193. doi: 10.1111/j.1365-2958.2012.08167.x

Figure 4. Specific histidine residues are important for NO· resistance and metal binding in STM1808.

Figure 4

A. S. Typhimurium cultures were grown in LB with (open symbols) or without (closed symbols) the addition of 5mM Sper/NO and growth monitored by measuring OD600 for 24h. Wild-type (black circles), ΔSTM1808 (purples squares), STM1808-H31A (red up triangles), STM1808-H32A (light purple down triangles), STM1808-H82A (light blue diamonds), STM1808-H95A (yellow diamonds) and STM1808-H102A (green squares). B. Metal determination of purified GST, GST-STM1808 and GST-STM1808-H82A by ICP-MS. Values represent three independent protein isolations. P-value was calculated using the Wilcoxon Rank-Sum Test.