In situ hybridization for selected differentially expressed and control genes, showing expression for: caudal C12 (A), caudal C13 (B), and rostral C13 (C) SOX11; caudal C13 (E) and rostral C13 (F) MAZ; rostral C13 GJA (G), caudal C13 VIM (H) and ISL1 (I); rostral C13 TBX3 (J), and caudal C13 CTNNB1 (K). Representative background staining shown for rostral C13 SOX11 (D) and caudal C13 VIM (L). Labels: da, dorsal aortae; di, diencephalon; fg, foregut; h, heart; lv, liver; nt, neural tube; ncc, neural crest cells; pa1–3, pharyngeal arches 1–3.