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. Author manuscript; available in PMC: 2013 Mar 6.
Published in final edited form as: Nature. 2012 Sep 6;489(7414):160–164. doi: 10.1038/nature11311

Figure 3. Expression of Nur77-GFP Bac Tg reporter is sensitive to genetic modulation of BCR signal strength and antigen.

Figure 3

(A) CD45 allelic series (low to high CD45 expression: L/L, L/+, +/+, H/−) GFPHI Tg splenic B cells were stained to identify B cell subsets as in Figure 2B, C. Overlaid histograms represent GFP expression in splenic subsets as gated in S5A.

(B) CD45+/+ GFPHI Tg and H/- (high CD45) GFPHI Tg splenic B cells with unrestricted (IgHEL-) or restricted (IgHEL+) repertoire in the absence of sHEL antigen were analyzed as in 3A. Overlaid histograms represent GFP expression in splenic subsets as gated in S5C.

(C) CD45+/+ GFPHI Tg splenic B cells with unrestricted (IgHEL-) or restricted (IgHEL+) repertoire in the presence or absence of sHEL antigen were analyzed as in 3A. Overlaid histograms represent GFP expression in splenic subsets as gated in S5D. All animals in Figure 3 were generated through genetic crosses. All data are representative of at least five independent experiments.