Fig. 2.

CysLT₁ receptor expression in human coronary artery smooth muscle cells is upregulated by pro-inflammatory stimuli. Real-time quantitative TaqMan RT-PCR for CysLT receptor mRNA in SMCs incubated in the absence and presence of LPS (10 μg/mL) for 1, 4, and 8 h (a) and IL-6 (20 ng/mL), TNF-α (10 ng/mL), or IFN-γ (20 ng/mL) for either 8 h (b) or 24 h (c). Results are expressed as fold increase compared with untreated cells (n = 3–5). *P < 0.05 vs. time-matched control