Fig. 5.

LTC₄-induced upregulation of PAI-2 in human coronary artery SMC. a Real-time quantitative RT-PCR for PAI-2 mRNA in human coronary artery SMC incubated in the absence and presence of LTC₄, LTD₄ or LTE₄ (1 μM) for 24 h. In some experiments, cells were pretreated with the CysLT₁ receptor antagonist MK571 (1 μM) for 1 h before addition of LTC₄. *P < 0.05 vs. non-LTC₄ stimulated Contr (n = 3–6). b PAI-2 concentrations in supernatants from human coronary artery SMC incubated 24 h in the absence (Contr) or presence of LTC₄ (1 μM). *P < 0.05 vs. Contr (n = 7). c Increase in PAI-2 mRNA levels induced by LTC₄ (1 μM) in human coronary artery SMC incubated in the absence (Contr) or presence of either BAPTA-AM or EGTA. *P < 0.05 vs. Contr (n = 3–6)