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. 2012 Sep 11;3:100. doi: 10.3389/fendo.2012.00100

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Copyright © 2012 Couturier and Deprez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Expected results from a P2I2 BRET-based screening assay. (A) Different expected BRET change upon inhibitor or modulator compound action compared to basal BRET signal. X and Y: Protein X and Y; D: energy donor; A: energy Acceptor; S: BRET Substrate. (B) Results of a 320 compounds miniscreen using OBRc/OBRGRP (●) and CD4/PLSCR1 (○) BRET-based screening assays, expressed as% of basal BRET in absence of compound in each plate. (C) Fluorescence measured from the same plates as in (B) expressed as % of fluorescence value in absence of compound (represented by ).

Inline graphic — Expected results from a P2I2 BRET-based screening assay. (A) Different expected BRET change upon inhibitor or modulator compound action compared to basal BRET signal. X and Y: Protein X and Y; D: energy donor; A: energy Acceptor; S: BRET Substrate. (B) Results of a 320 compounds miniscreen using OBRc/OBRGRP (●) and CD4/PLSCR1 (○) BRET-based screening assays, expressed as% of basal BRET in absence of compound in each plate. (C) Fluorescence measured from the same plates as in (B) expressed as % of fluorescence value in absence of compound (represented by ).