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. 2012 Apr 27;19(10):1655–1663. doi: 10.1038/cdd.2012.47

Figure 3.

Figure 3

Ethanol-induced cell death is unaffected by suppression of alcohol-metabolizing enzymes. (a) Kc 167 cells treated with dsRNAs targeting luciferase (an irrelevant control), Dronc, Adh and/or Adhr and vehicle (water) were challenged with 1% ethanol. (a, inset) Semiquantitative RT-PCR detects relative transcript levels of Adh and Adhr in dsRNA-treated cells as indicated. (b) Kc 167 cells treated with control dsRNAs targeting ampicilin-resistant gene (AmpR), Dronc, Aldh (two amplicons, kd1 and kd2) and vehicle (water) were challenged with 1% ethanol. (b, inset) Semiquantitative RT-PCR detects relative transcript levels of Aldh in dsRNA-treated cells as indicated. In both panels, cell survival was measured with CellTiter-Glo and normalized to untreated cells (mean±S.D., n=3)