Figure 5.

Drat is required for alcohol-induced cell death in both Kc cells and S2 cells. Kc 167 cells (a) or S2 cells (b) in 96-well microplates treated with 1.0 μg dsRNAs targeting luciferase or ampicilin, Dronc, Drat or vehicle (no dsRNAs) were challenged with 2% ethanol for 3 days, and cell survival was measured with CellTiter-Glo. Two distinct non-overlapping dsRNAs targeting Drat (kd1 and kd2) were tested here. Data was normalized to cells that were not challenged with ethanol (mean±S.D., n=3). (a, inset) Semiquantitative RT-PCR detects relative transcript levels of Drat and rp49 in cells treated with dsRNA in parallel as in the experiment