Figure 3.

Loss of Caspase-2 relieves the pressure to lose p53. (a) Representative western blot analysis of p19^ARF and p53 expression in tumors derived from Eμ-Myc, Eμ-Myc/Pidd^−/− and Eμ-Myc/Casp2^−/−. GAPDH serves as a loading control. In the last lane, a lysate derived from Eμ-Myc/Casp2^−/− (B10) or Eμ-Myc lymphoma (E91) is loaded as positive control. (b) Kaplan–Meier analysis of lymphomagenesis in mice with normal p53 or modified p53 status (mutated or lost, as assessed by western blotting in a). (c) Cohorts of Eμ-Myc/p53^+/− (n=5), and Eμ-Myc/p53^+/−/Casp2^−/− (n=5) mice were monitored for the development of B-cell lymphomas over time and data are depicted as tumor-free survival in a Kaplan–Meier analysis