Figure 5.

Lack of Pidd or Caspase-2 leads to a deregulation of cell cycle in Eμ-Myc tumors. (a) Cell cycle-distribution is depicted with G1 phase (white), S phase (light gray) and G2/M phase (dark gray) in tumor cells freshly isolated from Eμ-Myc (n=23), Eμ-Myc/Pidd^−/− (n=8) and Eμ-Myc/Casp2^−/− (n=13). (b) Tumor cells of wt Eμ-Myc (n=17), Eμ-Myc/Pidd^−/− (n=8) and Eμ-Myc/Casp2^−/− (n=13) were co-stained for mitosis-marker pH3 and PI. Bars represent percentages of pH3-positive cells ±S.E.M. *P<0.05. (c) Representative western blot analysis quantifying Caspase-2, Survivin or Lys⁹ pan-methyl H3 (mH3) levels in IgM⁺ tumors derived from Eμ-Myc, Eμ-Myc/Pidd^−/− and Eμ-Myc/Casp2^−/−. Detection of p38 MAPK served as a loading control