Figure 2.

Effect of iptakalim on NO production and eNOS activity in HPAECs under hypoxia. HPAECs were pre-treated with 0.1, 10 or 1,000 μM iptakalim for 1 h prior to hypoxia and were cultured under hypoxic conditions for 24 h. (A) NO levels and (B) eNOS activity were measured in the conditioned medium from HPAEC cultures as described in Materials and methods. Each value is the mean ± SD (n=6). ^*P<0.05 vs. normoxia; ^$P<0.05 vs. hypoxia. NO, nitric oxide; eNOS, endothelial nitric oxide synthase; IPT, iptakalim; HPAECs, human pulmonary artery endothelial cells.