Figure 5.
Western blot analysis of CHOP protein levels in glioblastoma cells treated with thapsigargin (1 μM) or tunicamyicin (1 μM). A172 cells (lanes 1–5); U373 cells (lanes 6–10). Vehicle-treated control cells (DMSO) (lanes 1 and 6); thapsigargin treatment for 24 h (lanes 2 and 7); thapsigargin treatment for 48 h (lanes 3 and 8); tunicamycin treatment for 24 h (lanes 4 and 9); tunicamycin treatment for 48 h (lanes 5 and 10). The images shown are representative of 3 independent experiments performed in duplicate that displayed similar results. CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide.