Figure 2.

Exon skipping activity of Pip6-PMOs in differentiated mouse H2K mdx myotubes. H2K mdx myotubes were incubated with peptide-PMO conjugates at concentrations ranging between 0.125 and 1 µmol/l without the use of transfection reagent. The products of nested reverse transcription-PCR (RT-PCR) were examined by electrophoresis on a 2% agarose gel. Exon skipping activity is presented as the percentage of Δ23 skipping as calculated by densitometry. PMO, phosphorodiamidate morpholino oligonucleotide.