Table III.

sMUC1 and anti-MUC1 autoantibody levels in multiple myeloma patients.

Patient no.	Patient status	sMUC1 TRA (titer)a	sMUC1 SP (titer)	Anti-MUC1 TRA Ab (μg/ml)b	Anti-MUC1 SP Ab (μg/ml)b	Anti-MUC1 TRA Ab (Positivity)	Anti-MUC1 SP Ab (Positivity)
1	Active disease; under therapy	Negative	Negative	440	740	+	+
2	Progressive disease; under therapy	Negative	Negative	183	150	-	-
3	At best response; off therapy	1:10	Negative	382	312	+	+
4	Active disease; under therapy	1:5	Negative	190	350	-	+
5	Active disease; under therapy	1:10	Negative	85	50	-	-
6	Active disease; under therapy	Negative	Negative	97	130	-	-
7	At best response; off therapy	1:10	Negative	355	456	+	+
8	At best response; off therapy	Negative	Negative	211	581	-	+
9	Active disease; under therapy	Negative	Negative	132	1036	-	+
10	At best response; off therapy	Negative	Negative	158	746	-	+
11	Progressive disease; under therapy	Negative	Negative	238	525	-	+
12	Progressive disease; under therapy	Negative	Negative	63	500	-	+
13	At best response; off therapy	Negative	Negative	500	1000	+	+
14	Active disease; under therapy	1:10	Negative	292	884	+	+
15	Progressive disease; under therapy	1:40	Negative	980	3400	+	+
16	At best response; off therapy	Negative	Negative	761	1500	+	+
17	Progressive disease; under therapy	1:20	Negative	91	100	-	-
18	Progressive disease; under therapy	Negative	Negative	728	424	+	+
19	Progressive disease; under therapy	1:10	Negative	795	339	+	+
20	Progressive disease; under therapy	Negative	Negative	120	80	-	-
21	Progressive disease; under therapy	1:20	Negative	3200	812	+	+
22	Progressive disease; under therapy	Negative	Negative	291	594	+	+
23	Progressive disease; under therapy	Negative	Negative	500	732	+	+
24	Progressive disease; under therapy	1:10	Negative	500	191	+	-
25	Progressive disease; under therapy	1:20	Negative	860	950	+	+
26	Active disease; under therapy	1:20	Negative	195	162	-	-
27	Progressive disease; under therapy	1:5	Negative	222	976	-	+
Percentage of patients with positive anti-MUC1 SP- and MUC1 TRA-specific antibodies levels						14/27	20/27

^aSerum levels of MUC1 antigen and anti-MUC1 antibodies were measured by ELISA assay as described in Materials and methods. Positive sMUC1 level is set for titer of X>1:5.

^bNaïve sera for anti-MUC1 TRA antibodies epitope is X≤274μg/ml and for anti-MUC1 SP autoantibodies is X≤210.2μg/ml, based on the average plus standard deviation values determined in 15 naïve healthy individuals.