Figure 4.
Involvement of PI3K in TRPC-mediated Ca2+ influx. A, Changes in fluorescence amplitude (Fluo-4/Fura Red) in NPCs exposed to PDGF-BB in the absence or presence of LY294002 (1 μm). B, Changes in fluorescence amplitude (Fluo-4/Fura Red) in NPCs exposed to PDGF-BB in the absence or presence of the indicated drugs (LY294002, 1 μm; calcicludine, 200 nm[scap]; NiCl2, 50 μm). Voltage-dependent channel blockers include calcicludine (L-, N-, P-type VDCC) and NiCl2 (T-type VDCC). All the data are presented as mean ± SD of four individual experiments. ***p < 0.001 versus control group; ###p < 0.001 versus PDGF-treated group; +p < 0.05 versus both PDGF-BB-treated and voltage-dependent channel blocker (calcicludine and NiCl2)-treated groups.