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Excitation and emission spectra of thiol adducts derived from 1a with various thiol compounds and cell homogenates. The thiol adducts’ maximum excitation and emission wavelengths for all tested compounds or cell homogenates are 430 and 520 nm respectively. The spectra were obtained on a FS900 spectrofluorometer and from a sample (3 mL) by mixing a thiol compound (20 µM) or cell homogenates (NCI-H226, 2×106 cells) with 1a (10 µM) in Tris buffer (0.1 M, pH 7.0) for 10 min.
