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. Author manuscript; available in PMC: 2013 Aug 7.
Published in final edited form as: Anal Chem. 2012 Jul 26;84(15):6877–6883. doi: 10.1021/ac301306s

Figure 4.

Figure 4

Emission spectra of sulfide 1a and the reaction mixtures of 1a with various amino acids. 1a (0.1 mM, 150 µL) in acetonitrile was mixed with an equal volume of a solution of an amino acid [5 mM in Tris buffer (0.1 M, pH 7.0) containing 2 mM EDTA], except NAC methyl ester which was made a solution of 0.1 mM, in a 96-well plate. The mixture was allowed to stand at room temperature for 10 min before subjected to fluorescence analysis. The emission spectra were obtained with the excitation wavelength at 430 nm on a SpectraMax M2 Microplate Reader. The results are expressed as the fluorescence intensity and representatives of a triplicate.