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Rapamycin disrupts mTORC2 in myotubes. mTORC2 was immunoprecipitated from myotubes that were pre-treated with vehicle, rapamycin for 1 h, or rapamycin for 24 h, using antibodies to Rictor (A) or mTOR (B). The integrity of the complex was assessed by blotting for the opposite subunit. In each case, mTORC2 formation was disrupted at 24 h despite only minor changes in total protein levels. The mTORC1 subunit Raptor was also absent from mTOR immunoprecipitates at either 1 or 24 h.
