Table 1.
Antibodies used in the study
| Antibody | Dilution | Source | Clone | Incubation | Instrumentation |
|---|---|---|---|---|---|
| p53+ | PDL* | Ventana;AZ | DO-7 | 24 min | BenchMark XT |
| PR+ | PDL* | Ventana;AZ | 1E2 | 20 min | BenchMark XT |
| ER+ | PDL* | Ventana;AZ | SP1 | 16 min | BenchMark XT |
| HNF-1b** | 1:100 | Santa Cruz Bio; CA | H-205 | 15 min | Leica Bond |
| BAF250a** | 1:100 | Sigma; MO | HPA005456 | 15 min | Leica Bond |
*
Denotes a pre-diluted antibody.
**
Antigen retrieval was performed with Bond Epitope Retrieval Solution 1 (Leica), a citrate buffer-based pH 6.0 solution for 20 minutes at 100 ° C.
+
Antigen retrieval was performed with Cell Conditioning 1 (Ventana), a tris-based buffer pH 8.3 solution for 30 minutes at 100°C.