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. 2011 Dec 28;3(3):437–442. doi: 10.3892/etm.2011.439

Figure 2.

Figure 2.

(A–C) Hoechst staining and FAM green fluorescence were used to detect the transfection efficiency. (A) Hoechst staining of cells 72 h following the transfection of A431 cells. (B) The green fluorescence of cells 72 h following the transfection of A431 cells. (C) The merge of the two types of fluorescence. The scale bar is 100 μm. (D) Gene silencing of the TCTP gene by siRNA transfection. A431 cells (104 cells/ml) were transfected with TPT1-272-siRNA, TPT1-316-siRNA, TPT1-351-siRNA or siRNA-NC Lipofectamine reagent only. A431 cells transfected with Lipofectamine only served as untreated control. mRNAs extracted from cells at 24, 48 and 72 h following the transfection were used to detect the expression of the TPT1 gene by RT-PCR. Results revealed that the expression of the TPT1 gene was significantly silenced by siRNA transfection; TPT1-351-siRNA had the best knockdown effect 72 h following transfection. TCTP, translationally controlled tumor protein; siRNA, small interfering RNA.