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. 2011 Nov 16;3(2):255–260. doi: 10.3892/etm.2011.381

Figure 1.

Figure 1.

Effect of micro30 shRNA on the mRNA of Cyclin D1 and Bcl-xL (mean ± SD). The plasmids were transfected into A549 and NCI-H441 cells. Gene expression values were then calculated. (A) Cyclin D1 mRNA expression was significantly reduced in A549 and NCI-H441 lung cancer cells transfected with Cyclin D1 shRNA and Cyclin D1-Bcl-xL shRNA (combined shRNA) compared with the blank control group, *P<0.05. (B) Bcl-xL mRNA expression was significantly reduced in A549 and NCI-H441 lung cancer cells transfected with Bcl-xL shRNA and Cyclin D1-Bcl-xL shRNA compared with the blank control, *P<0.05. No significant difference was found among the combined shRNAs and single shRNA on Cyclin D1 or Bcl-xL, respectively, in the transfected cells, P>0.05. SD, standard deviation; shRNA, short hairpin RNA; RT-PCR, reverse transcripion-polymerase chain reaction.