Figure 3.

Tumorsphere formation assay upon treatment of U87-MG cells with Trichostatin A (TSA). A seventy-two-hour treatment with the histone deacetylase inhibitor TSA affects tumorsphere formation and cellular proliferation after medium shift to NSC medium. Bar represents control percentage. *represents P < 0.02 for 100 nM and P < 0.001 for 300 and 500 nM for spheres count, and ^#represents P < 0.046 for 100 nM P < 0.011 for 300 nM and P < 0.001 for 500 nM for cell count as measured by trypan blue. One-way ANOVA, followed by Turkey's post hoc test were used for statistical analysis, where P-values <0.05 were considered significant.