. 2012 Sep;12(9):769–781. doi: 10.1089/vbz.2011.0766

Table 3.

Ixodid and Argasid Tick Cell Lines Tested for Ability to Support Crimean-Congo Hemorrhagic Fever Virus Replication

Tick species (role in virus transmission if known)	Cell line	Culture medium	Reference
Hyalomma anatolicum (vector)	HAE/CTVM8	L-15^a/H-Lac^b,c	Bell-Sakyi 1991
	HAE/CTVM9	L-15/MEM^d	Bell-Sakyi 1991
Amblyomma variegatum	AVL/CTVM13	L-15/L-15B^e	Bell-Sakyi et al. 2000
Rhipicephalus (Boophilus) decoloratus	BDE/CTVM14	H-Lac	Lallinger et al. 2010
Rhipicephalus (Boophilus) microplus	BME/CTVM2	L-15	Bell-Sakyi 2004
	BME/CTVM6	L-15	Bell-Sakyi 2004
Ixodes ricinus	IRE/CTVM20	L-15/L-15B	Lallinger et al. 2010
Rhipicephalus appendiculatus	RAE/CTVM1	L-15	Bell-Sakyi 2004
Ornithodoros moubata	OME/CTVM21	L-15/H-Lac	Bell-Sakyi et al. 2009
	OME/CTVM22	L-15/H-Lac	Bell-Sakyi et al. 2009

^{^a}

L-15: Leibovitz medium supplemented with 10% tryptose phosphate broth (TPB), 20% fetal calf serum (FCS), 2 mM l-glutamine (L-g), 100 units/mL penicillin, and 100 μg/mL streptomycin (p/s).

^{^b}

Hank's balanced salt solution supplemented with 0.5% lactalbumin hydrolysate, 20% FCS, L-g, and p/s.

^{^c}

A 1:1 mixture of L-15 and H-Lac.

^{^d}

A 1:1 mixture of L-15 (Leibovitz) and minimal essential medium with Hank's salts supplemented with 10% TPB, 20% FCS, L-g, and p/s.

^{^e}

A 1:1 mixture of L-15 and L-15B (Munderloh and Kurtti 1989) supplemented with 10% TPB, 5% FCS, 0.1% bovine lipoprotein concentrate (MP Biomedicals), L-g, and p/s.

All tick species are ixodid except O. moubata. Cell lines were maintained in flat-sided tubes (Nunc) at 31°c with weekly medium changes (Bell-Sakyi 1991, 2004, Bell-Sakyi et al. 2000, 2009, Lallinger et al. 2010). All medium components were obtained from Sigma-Aldrich except where indicated. Before infection with Crimean-Congo hemorrhagic fever virus, cells were seeded in flat-sided tubes at 2×10⁵ cells/mL in 2 mL of appropriate culture medium.