Table 1.
Individual Collected Samples of Bone Marrow-Derived Mesenchymal Stromal Cells Used in This Study and Mesenchymal Stromal Cells with Limited In Vitro Growth
| Subject | Gender | Age (y.o) | Observations | MSC passage | CD34+ (%)a | EDb |
|---|---|---|---|---|---|---|
| N10 | F | 26 | MO and MOSN;c functional | p3-p5 | 1.80 | +14 |
| N14 | M | 22 | MO and MOSN;c functional | p2-p5 | 1.70 | +15 |
| N15 | M | 29 | MO and MOSN;c functional | p2-p3 | 1.40 | +13 |
| N20 | M | 27 | MO and MOSN;c functional | p4 | 1.20 | +14 |
| N22 | M | 24 | MO and MOSN;c functional | p2-p4 | 0.90 | +14 |
| N34 | M | 33 | MO and MOSN;c functional | p5 | 1.30 | +15 |
| C38 | M | <40 | MO and MOSN;c functional | p2-p3 | ND | ND |
| N11 | M | 32 | MO and MOSN;c non-functional | p2 | 0.80 | +18 |
| N7 | M | 22 | dMOSN; limited growth. Donor presented leucopenia 1 year before harvesting | p2 | 0.80 | +20 |
| N9 | M | 32 | dMO; limited growth | p2 | 1.50 | +15 |
| N13 | M | 25 | dMOSN; limited growth | p2 | 0.90 | +19 |
| N18 | M | 34 | dMO; limited growth | p1 | 0.58 | +14 |
| N24 | M | 27 | dMO; limited growth | p2 | 2.20 | +13 |
| N25 | M | 23 | dMO; limited growth | p3 | 1.90 | +14 |
| N26 | M | 40 | dMO; limited growth | p1 | 1.30 | +11 |
| N27 | M | 34 | dMO; limited growth | p2 | 1.40 | +13 |
| N28 | M | 34 | dMO; limited growth | p1 | 0.90 | +16 |
| N29 | M | <40 | dMO; and MOSN; limited growth | p1 | ND | ND |
| N30 | M | 29 | dMO; and MOSN; limited growth | p1 | 0.98 | +13 |
| N31 | M | 28 | dMO; limited growth | p1 | 1.10 | +15 |
| N33 | M | 27 | dMO; and MOSN; limited growth | p3 | 1.50 | +18 |
| N36 | M | 34 | dMOSN; limited growth | p1 | 1.10 | +14 |
Samples presented no technical impairments at time of collection. Samples presenting blood coagulation or other technical impairments were not included in this table.
Percentage of CD34+ cells in the individual BM sample (donor).
Engraftment day (ED) as+number of days with >500 neutrophils, >2×104 platelets/mm3, and hemoglobin >8 gr/dL, without transfusion requirements (BM recipient).
In vitro assays with MSCs collected from BM corresponding to individual donors. MSCs from individual donor samples were classified into functional and non-functional according to their in vitro assays responsiveness to chemotactic assays.
MO- and/or MOSN-MSCs from individual BM samples with limited in vitro growth, which did not allow to collect enough number of cells for experimental assays.
MSC, mesenchymal stromal cells; BM, bone marrow; y.o., years old; p, passage; ND, no data; M, masculine; F, feminine.