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. Author manuscript; available in PMC: 2012 Sep 11.
Published in final edited form as: Oncogene. 2009 Jul 6;28(38):3380–3389. doi: 10.1038/onc.2009.193

Figure 4.

Figure 4

As P21 gene expression is upregulated by human immunodeficiency virus 1 (HIV-1) infection, COUP-TF-interacting protein 2 (CTIP2) inhibits HIV-1 replication in human primary macrophages and microglial-like cells. (a) RNAs from HIV-1/Bal-infected cells were extracted and assessed for p21 mRNA expression. Inductions at each time points are presented relative to the control glyceraldehyde 3-phosphate dehydrogenase level. (b) Cells were transfected with HIV-1 pNL4-3 in the presence or absence (control cells) of the Flag-CTIP2 expression vector. HIV-1 reverse transcriptase activity was assessed 24-, 48- and 72-h post-transfection. Inhibition of HIV-1 replication is presented relative to the reverse transcriptase (RT) activity detected with the HIV-1-infected control cells. RT activity in control cells (without Flag-CTIP2) varied between 0 and 197 pg/ml.