Table 1.
primers used for constructing synthetic splice acceptor
| Primer name | Primer sequence 5’ – 3’ |
|---|---|
| SAF | CTCGAGGACTATCCGGAGTGTGTGTGTGTTTCTGACGTGTCTCTCTCTTCCCTTTTTTTTTTCAGGTCGACAAACTCTTCG |
| SAR | CCATGGTGGTTCTTCTCTTCTTCCGACCGCGAAGAGTTTGTCGACCTG |
| LM-PCR Long | CTAGGATTAGCTGCTGGAGTACACGATCGCTTAATAGAGGCACGTGGAACGCGGGC |
| LM-PCR Short CG | PCGGCCCGCGTTCCACGTGZZOG |
| LM-PCR long CATG | CTAGGATTAGCTGCTGGAGTACACGATCGCTTAATAGAGGCACGTGGAACGCGGGCCGCATG |
| LM-PCR UP1 | CTAGGATTAGCTGCTGGAGTACACG |
| LM-PCR NUP2 | ATCGCTTAATAGAGGCACGTGGAAC |
| 5’ TIR1 | CCAAAGGACCAATGAACATGTCTGAC |
| 5’ TIR2 | AACTGGGCATCAGCGCAATTCA |
| 5’ TIR3 | TTGTACTCAAGTAAAGTAAAAATCCC |
| 3’ TIR1 | TCAGCCCCAAAAGAGCTAGGCTTG |
| 3’ TIR2 | GCGTGTACTGGCATTAGATTGTCTGTC |
| 3’ TIR3 | TCAAGTAAGATTCTAGCCAGATAC |
| GT1 | CTTCCGACCGCGAAGAGTTTGTC |
| GT2 | CTTCTAACTATAACGGTCCTAAGGTAGCG |