5.0 µl | 5× GoTaq buffer (Promega) |
3.0 µl | 25 mM MgCl2 |
5.0 µl | DNA with linker |
0.5 µl | 10mM dNTPs |
0.5 µl | 10 µM UP1 |
0.5 µl | 10 µM 5’TIR1 |
10 µl | H20 |
0.2 µl | Taq DNA polymerase (5U/µl) |
5.0 µl | 5× GoTaq buffer (Promega) |
3.0 µl | 25 mM MgCl2 |
5.0 µl | DNA with linker |
0.5 µl | 10mM dNTPs |
0.5 µl | 10 µM UP1 |
0.5 µl | 10 µM 5’TIR1 |
10 µl | H20 |
0.2 µl | Taq DNA polymerase (5U/µl) |