Skip to main content
. Author manuscript; available in PMC: 2012 Sep 11.
Published in final edited form as: Methods Cell Biol. 2011;104:1–22. doi: 10.1016/B978-0-12-374814-0.00001-X
10.0 µl 5× GoTaq buffer (Promega)
6.0 µl 25 mM MgCl2
1.0 µl Diluted 1st round product
1.0 µl 10mM dNTPs
1.0 µl 10 µM NUP2
1.0 µl 10 µM 5’TIR2
31.0 µl H20
0.2 µl Taq DNA polymerase (5U/µl)