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. Author manuscript; available in PMC: 2012 Sep 11.
Published in final edited form as: Methods Cell Biol. 2011;104:1–22. doi: 10.1016/B978-0-12-374814-0.00001-X
5.0 µl 5× GoTaq buffer (Promega)
3.0 µl 25 mM MgCl2
5.0 µl DNA with linker
0.5 µl 10mM dNTPs
0.5 µl 10 µM UP1
0.5 µl 10 µM 3’TIR1
10 µl H20
0.2 µl Taq DNA polymerase (5U/µl)