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. 2012 Jul 24;287(37):30993–31002. doi: 10.1074/jbc.M112.376186

FIGURE 1.

FIGURE 1.

Covalent dimerization of MyD88 TIR domains improves TLR inhibition. A, Structural model of MyD88 TIR dimer based on the crystal structure of dimeric TLR10 TIR domains including the 25 amino acid linker (dashed line). B–E, improved inhibition of TLRs with dTIR in comparison to mTIR on HEK293 cells. B, cells were transfected with TLR4 and MD-2 plasmids (1 ng) and mTIR or dTIR plasmids (1, 5, 10 ng) and stimulated with S-LPS (10 ng/ml). C, cells were transfected with TLR5 (20 ng) and mTIR or dTIR plasmids (1, 5, 10 ng) and stimulated with flagellin (10 ng/ml). D, cells were transfected with TLR9 (10 ng) and Unc93B1 plasmids (1 ng) and mTIR or dTIR plasmids (1, 10, 20 ng) and stimulated with ODN (3 μm). E, cells were transfected with TLR3 (20 ng) and mTIR or dTIR plasmids (1, 5, 10 ng) and stimulated with poly(I:C) (10 μg/ml). Activation of TLR signaling pathway was determined by the dual luciferase assay. Representative graphs are shown from three separate experiments. Data are represented as mean ± S.D.