FIGURE 3.

VacA induced generation of LC3-II in an LRP1-dependent manner. a, AZ-521 cells were incubated with 120 nm heat-inactivated (IA) or wild-type VacA (A) for the indicated time points and harvested for immunoblotting (IB) with the indicated antibodies. Quantification of VacA-induced LC3-II levels in AZ-521 cells was performed by densitometry (bottom panel). Data are presented as mean ± S.D. of values from two experiments. Experiments were repeated two times with similar results. b, the indicated siRNA-transfected AZ-521 cells were incubated with 120 nm heat-inactivated or wild-type VacA for 4–5 h at 37 °C and the cell lysates were subjected to immunoblotting with the indicated antibodies. α-Tubulin served as a loading control. Quantification of VacA-induced LC3-II levels in AZ-521 cells was performed by densitometry (bottom panel). Data are presented as mean ± S.D. and significance is (*) p < 0.01 (n = 4). Experiments were repeated four times with similar results.