FIGURE 6.

Among VacA-binding proteins, LRP1, but not RPTPs and FN, mediates VacA-dependent autophagy. a, the indicated siRNA-transfected AZ-521 cells were incubated with 120 nm heat-inactivated (IA) or wild-type VacA (A) for 4–5 h at 37 °C and the cell lysates were subjected to immunoblotting (IB) with the indicated antibodies. The knockdown levels of RPTPβ or RPTPα were detected by the antibodies (right panel). α-Tubulin served as a loading control. b, the indicated NC or RPTPβ siRNA-transfected AZ-521 cells were incubated with 120 nm heat-inactivated or wild-type VacA for 4–5 h at 37 °C and the cell lysates were subjected to immunoblotting with anti-LC3B or anti-FN antibodies. α-Tubulin served as a loading control. c, AZ-521 cells were transfected with NC or FN siRNA and treated with heat-inactivated or wild-type VacA for 4–5 h at 37 °C. Cell lysates were subjected to immunoblotting with the indicated antibodies. α-Tubulin served as a loading control. A blot representative of at least three separate experiments is shown.